Verificação possibilidade de determinação de espironolactona em presença de furosemida por espectrofotometria

Abstract

Furosemide (FUR) is used in the treatment of heart failure. As spironolactone (ESP) also potent diuretic drug of the class is used for cardiac insufficiency and hypertension. This association is valid because the two are complementary treatment. The structures and physicochemical properties of FUR and ESP are similar, giving absorption at a wavelength near generating inference, hindering the determination of the components in the mixture. Where lack of official methodologies, methodological alternatives are used for content. The derivative spectrophotometry first order (EDPD) and multiple linear regression by least squares classics (CLS) are the usual for multicomponent mixtures. Methanolic solutions of standards were prepared from 2 for the 10μg.mL-1 furosemide and 5 to 25μg.mL -1 CLS to calibrate the system and to generate calibration curves for EDPD. Was measured in the region of 200 nm to 400 nm adapted addressed data into Excel spreadsheet. In EDPD gave points for annulment in 248 spironolactone 351 nm furosemide. The calibration solutions were tested in CLS and EDPD, the recovery rate is consistent with expected. The results for the standards were similar to those in the literature. However the sample results analyzed showed a value of ESP lower than expected, requiring analysis of other drugs.