Estabelecimento de sistema de expressão heteróloga para a bioprospecção de genes de interesse biotecnológico

Abstract

The wide variety of environments and ecosystems represents a wide variety of genera and species of bacteria that still remain largely unknown or can not be cultured. This biodiversity represents an enormous wealth in terms of microorganisms and metabolic processes that can be directed to bioprospecting of genes of biotechnological interest. However few studies involving functional analysis of these communities isolated new genes. Many difficulties are associated with heterologous expression systems employed. In this study we report the construction of a BAC vector for cloning and heterologous expression based on the plasmid-RK2 containing promoters with dual guidance system of regulating the number of copies, as well as mechanisms for the transfer and maintenance in different hosts such as Escherichia coli and Bacillus subtilis.